February 2021 Spray 23
ment from the individual test values. This helped to account for the
variations in moisture content seen across the surface of the hand
from each site. The moisture studies were conducted in a room set
at 22–25°C (71.6–77°F) and 50–60% relative humidity (RH).
While the products were designed to be sprays, for the purposes
of these studies, the products were applied by saturation
of a cotton swab and application
of the formulations directly to the
back of the hand at the appropriate
site. This helped to keep the
amount of product applied to
each site very uniform. After application,
the volunteers allowed the
products to dry and then sat for
15 minutes whereupon hydration
measurements were made again
and then the products reapplied.
This was done for a series of six
time points over a 90-minute time
frame.
Alcohol-Free Hand Sanitizing
Foam Moisturization Study
In the studies employing alcoholfree
hand sanitizing foams, a commercially
available Benzalkonium
Chloride-based hand sanitizing
foaming product was employed.
To this commercial formula were
added sequentially increasing
concentrations of BotaniDew NMF
including 0.25%, 0.5% and 1.0%.
The placebo formulation was the
commercial product used as is.
While the products are designed to
foam when delivered from a pump or aerosolized delivery system,
for the purposes of these studies, the products were applied to
the back of the hand using cotton swabs to control the amount of
product applied. A separate cotton swab was used for each sample
and the swabs were replaced after a single use so that components
from the formulations could not accumulate on the swabs.
A total of 10 individual hands
were examined in this moisturization
study using the same testing conditions
described above. However,
because four products were being
tested, the control in this study included
four untreated test sites near
the knuckles of the hand and four
untreated test sites near the wrist
that were then averaged together.
Results
Hand Sanitizer Spray
Moisturization Study Results
The results of the hand moisturizing
studies employing the glycerinbased
hand sanitizing sprays on four
volunteers’ hands can be seen in
Figure 2. The results of the hand
sanitizing studies employing the
Glycereth-26 formulations can be
seen in Figure 3.
Hand Sanitizer Foam
Moisturization Study Results
The results of the hydration studies
employing the hand sanitizing
foams can be seen in Figure 4.
Figure 2: Results of skin moisturizing studies (in μS) for glycerin-based moisturizing formulations containing 3%
and 5% BP-BotaniDew NMF and corresponding placebo formulation replacing BP-BotaniDew NMF with 4%
water. Data also includes untreated control site (p≤0.05).
Figure 3: Results of skin moisturizing studies (in μS) for glycereth-26-based moisturizing formulations containing
3% and 5% BP-BotaniDew NMF and corresponding placebo formulation replacing BP-BotaniDew NMF with
4% water. Data also includes untreated control site (p≤0.05).